FIGURE

Fig. 7

ID

ZDB-FIG-141023-15

Publication

Alexander et al., 2014 - Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton

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Fig. 7

Bmp and Edn1 restore ventral-intermediate gene expression, but not hand2, in dntcf3+ embryos.

(A–P) Whole mount ISH for ventral-intermediate patterning genes in heat shocked controls (A, E, I, M), dntcf3+ embryos (B, F, J, N), and dntcf3+ embryos with either 25 ng BMP4/7 (C, G, K, O), or 5 ng EDN1 (D, H, L, P), lateral views, anterior to the left. Asterisks in G and K indicate cases in which rescue occurred in arch 1 but not arch 2. (Q) Histogram quantifying the number of embryos with reductions in arch expression for each gene. Quantification obtained by counting number of embryos with rescue of gene expression in three independent experiments (minimum of 10 embryos per protein injection experiment). Statistical method used is a paired student t-test. * P<0.05, ** P<0.001. Scale bar: 100 µm.

Expression Data

Genes:	dlx3b dlx5a hand2 msx1a
Fish:	WT w26Tg/+
Condition:	heat shock
Anatomical Terms:	pharyngeal arch pharyngeal arch ventral region
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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