Fig. S7

Sox4 compensates for the loss of Sox11. (A) Sox4a was diffusely expressed in the control retina at 36 hpf (left); however, sox4a expression was upregulated in the lens and retina of sox11 morphants (right; n = 20 per group); scale bar = 50 µm. (B) QPCR performed on mRNA from the heads of 24 hpf zebrafish embryos injected with sox11 MO or control MO reveal that sox4a expression is elevated in sox11 morphants compared to controls. Relative transcript abundance was normalized to gapdh levels and is presented as the mean fold-change in expression relative to controls (n = 40 embryos per group, 3 independent biological repeats) *p<0.01, Student′s t-test. (C) Co-injection of sox4 mRNA rescued the lens and coloboma phenotypes of sox11 morphants at 24 hpf and 2dpf. Number of embryos analyzed: 24 hpf control MO, n = 136; 2 dpf control MO, n = 124; 24 hpf sox11 MO, n = 179; 2 dpf sox11 MO, n = 161, 24 hpf sox11 MO + sox4 mRNA, n = 210, 2 dpf sox11 MO + sox4 mRNA, n = 184, 3 independent biological replicates. *p<0.001, Fishers exact test; MO, morpholino.