FIGURE

Fig. 2

ID

ZDB-FIG-140908-12

Publication

Majumdar et al., 2000 - The zebrafish floating head mutant demonstrates podocytes play an important role in directing glomerular differentiation

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Fig. 2

Glomerular defects in sonic you and you-too mutants correlate with failed sclerotome development. Transverse section of a 48-hpf syu^tbx392/t4 embryo (A) reveals the absence of a midline glomerulus and in its place are two lateral cell clusters (arrows). In situ hybridization of syu^tbx392/t4 embryos with a wt1 probe (B) identifies the lateral cell clusters as podocytes. Similar histological section of a 36-hpf you-too embryo (C) shows a failed convergence of podocytes. In situ hybridization with gli2 probe (D) shows expression in the neural tube and fin bud (dark arrows) but no expression in the pronephric primordia (light arrow). Transverse histological section through a wildtype 3-week-old zebrafish (E) shows the glomerulus (gl) is surrounded by bone and cartilaginous tissue (double arrows; da, dorsal aorta). twist-positive cells are found in the wildtype ventromedial somite at 18 hpf (F). twist-positive cells are absent in flh mutants (G). Pax9-expressing cells are found between neural tube and somite tissue in 24-hpf wildtype embryos (H). Pax9-expressing cells are reduced in flh (I). Scale bar equals 50 µm.

Expression Data

Genes:	gli2a pax9 twist2 wt1a
Fish:	WT noto^n1/n1 (AB) shha^tbx392/+; Df(Chr07)t4/+
Anatomical Terms:	brain neural tube pectoral fin bud podocyte somite somite ventro-medial region
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gli2a^ty119/ty119 noto^n1/n1 (AB) shha^tbx392/+; Df(Chr07)t4/+
Observed In:	cell migration involved in kidney development podocyte pronephric glomerulus sclerotome
Stage Range:	14-19 somites to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 222(1), Majumdar, A. and Drummond, I.A., The zebrafish floating head mutant demonstrates podocytes play an important role in directing glomerular differentiation, 147-157, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.