PUBLICATION
The zebrafish floating head mutant demonstrates podocytes play an important role in directing glomerular differentiation
- Authors
- Majumdar, A. and Drummond, I.A.
- ID
- ZDB-PUB-000824-1
- Date
- 2000
- Source
- Developmental Biology 222(1): 147-157 (Journal)
- Registered Authors
- Drummond, Iain, Majumdar, Arindam
- Keywords
- zebrafish; floating head; pronephros; glomerulus; podocyte; sclerotome
- MeSH Terms
-
- Animals
- Cell Differentiation/genetics*
- Embryo, Nonmammalian/cytology
- Homeodomain Proteins/genetics*
- Kidney Glomerulus/cytology*
- Kidney Glomerulus/ultrastructure
- Microscopy, Electron
- Mutation*
- Transcription Factors/genetics*
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 10885753 Full text @ Dev. Biol.
Citation
Majumdar, A. and Drummond, I.A. (2000) The zebrafish floating head mutant demonstrates podocytes play an important role in directing glomerular differentiation. Developmental Biology. 222(1):147-157.
Abstract
In zebrafish, the pronephric glomerulus occupies a midline position underneath the notochord and is vascularized through angiogenic capillary ingrowth from the dorsal aorta. The midline mutants floating head (flh), sonic you (syu), and you-too (yot) provide the opportunity to study glomerular differentiation in the absence of the notochord and vascularization from the dorsal aorta. In flh, syu, and yot mutants, glomeruli differentiate at ectopic lateral positions within the embryo and contain morphologically identifiable podocyte and endothelial cell types. In the absence of the dorsal aorta, endothelia from an alternate source are recruited by podocytes during glomerular vascularization to make functional glomeruli. Our results suggest that midline signals are required for proper glomerular morphogenesis but not for the differentiation of podocytes. Podocytes appear to play an important role in directing cellular recruitment events leading to glomerular differentiation. Furthermore, we find defects in sclerotomal development that correlate with defects in glomerular morphogenesis suggesting a possible link between the formation of these embryonic structures.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping