FIGURE

Fig. 7

ID

ZDB-FIG-140812-6

Publication

Kotkamp et al., 2014 - Pou5f1/Oct4 promotes cell survival via direct activation of mych expression during zebrafish gastrulation

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Fig. 7

Mych overexpression and p53 knockdown suppress cell death in MZspg gastrulae.

TUNEL staining to detect apoptosis at bud stage (A-J) and subsequent computational image analysis (K) for quantification of the number of apoptotic cells. The images show maximum intensity projections of z-stacks taken from single embryos with dorsal to the right. WT embryos display almost no apoptosis, whereas MZspg mutants show an increase in cell death throughout the embryo (A-D). This mutant apoptosis phenotype was partially repressed either by mych overexpression (E,F) or p53 morpholino knockdown (G,H). The co-injection of mych mRNA and p53-morpholino could completely suppress cell death in MZspg mutants, but did not rescue the delay in epiboly movement (I-J; arrowheads). The quantification of cell death (K) revealed that the number of apoptotic cells is decreased by a factor of six in MZspg embryos after mych mRNA injection. By combined knockdown of p53 and Mych overexpression, apoptosis in MZspg embryos was reduced to WT levels.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pou5f3^m793/m793
Observed In:	apoptotic process epiboly
Stage:	Bud

Phenotype Detail

Acknowledgments

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