FIGURE

Fig. 5

ID

ZDB-FIG-140812-4

Publication

Kotkamp et al., 2014 - Pou5f1/Oct4 promotes cell survival via direct activation of mych expression during zebrafish gastrulation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Analysis of the Mych contribution to the morphology of the MZspg mutant phenotype.

Knockdown of Mych function in WT by injecting splice-blocking (A-D) or translation-blocking (E-H) morpholinos. Morphological analysis of injected (A,C,E,G) and non-injected (B,D,F,H) embryos at 90%-epiboly and 25 hpf. Morphants show neither a delay in epiboly (A, E) nor obvious morphological defects by 25 hours development (C, G). Rescue of Mych activity in MZspg mutants by injecting mych mRNA into 1-cell stage embryos (I-L). Mych alone is not able to rescue the strong defects developed by Pou5f1 deficient embryos during gastrulation (I) and after 25 hpf (K).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pou5f3^m793/m793 WT + MO3-mych WT + MO4-mych
Knockdown Reagents:	MO3-mych MO4-mych
Observed In:	epiboly whole organism
Stage Range:	90%-epiboly to Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One