Fig. 3

Quantitative analysis of the zebrafish axial skeleton by MS-based proteomics.

(A) Alcian blue/alizarin red stain of cartilage/bone structures in the axial skeleton. Lateral images of all three time point used for protein extraction (left to right: 14 dpf, 28 dpf, 358 dpf). (B) VENN diagram of proteins selected for label-free quantification, with areas drawn to represent the number of proteins. Total number of proteins as well as distinct and common proteins are indicated for each time point. Proteins that did not qualify for label-free quantification are depicted in grey. (C-E) Ratio abundance plots showing log total iBAQ intensities versus log protein abundance ratio of the 28/14 dpf (C), the 358/28 dpf (D) and the 358/14 dpf (E) axial skeleton ratios of all the proteins that met the strict criteria for label-free quantification (black circles, significantly differential abundant proteins at FDR = 0.01; dark grey circles, significantly differential abundant proteins at FDR = 0.05; light grey circles, no significant change in abundance). The numbers correspond to the proteins listed in 3F. (F) Table containing several of the significant differentially abundant proteins within the axial skeleton. Specific proteins are discussed in the text.