FIGURE

Fig. 1

ID

ZDB-FIG-140808-1

Publication

Monteiro et al., 2014 - V-ATPase proton pumping activity is required for adult zebrafish appendage regeneration

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Fig. 1

(A) Recording chamber for H⁺ flux measurement using SIET, representing zebrafish emerged in recording medium. (B) H⁺ efflux established during regeneration. *statistical significant results (n = 10, p<0.05). (C) Affimetrix microarray to assess gene expression during caudal fin regeneration, compared to intact fins. (D–I) In situ hybridization of V-ATPase subunits atp6v1e1b (D–F) and atp6v1a (G–I), at 24 hpa (D, G), 48 hpa (E–H) and 72 hpa (F–I). (J–M) Immunohistochemical detection of Atp6v1a at 24 hpa (J, K), 48 hpa (L) and 72 hpa (M). White arrowheads point to the Atp6v1a blastema localization. (J) Detail of Atp6v1a (green) cellular localization in blastema cells, 24 hpa. hpa: hours post amputation. For each panel, n = 6, except mentioned otherwise.

Expression Data

Genes:	atp6v1aa atp6v1e1b
Antibody:	Ab4-atp6v1a
Fish:	AB
Condition:	physical alteration: anatomical structure
Anatomical Terms:	caudal fin blastema regenerating fin lepidotrichium joint
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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