FIGURE

Fig. 4

ID

ZDB-FIG-140807-58

Publication

Ma et al., 2014 - The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish

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Fig. 4

Neurons and macrophages/microglia express Legumain in the caudal spinal cord after SCI.

Spinal cord sections from fish 3 days after SCI were used for double staining of Legumain and GFAP (A–D) or 4C4 (E–H). Spinal cord sections from fish 11 days after SCI were used for double staining of Legumain and NeuN (I–L). B, F, and J are the magnifications of A, E and I, respectively. No co-localization of Legumain and GFAP is observed (A–D). Double immunostaining of Legumain with 4C4 antibody identifies the positive small cells as macrophages/microglia (E–H). Double staining with NeuN shows that neurons express Legumain at 11 days after SCI (I–L). n = 3 experiments. Scale bar, 50 μm.

Expression Data

Antibodies:	Ab-4C4 Ab1-lgmn Ab1-rbfox3 Ab2-gfap
Fish:	WT
Condition:	transection: spinal cord
Anatomical Terms:	spinal cord astrocyte spinal cord macrophage spinal cord microglial cell spinal cord neuron
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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