FIGURE

Fig. 8

ID

ZDB-FIG-140807-39

Publication

Araya et al., 2014 - Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo

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Fig. 8

Neural cell movements in MZoep embryos are highly disrupted through neural keel to neural tube stages. (A,B) Selected frames from time-lapse sequences showing normal neurulation in a wild-type embryo and disrupted neurulation in a MZoep embryo from 12 hpf. Cells are labeled with H2B-RFP to reveal movements of nuclei (gray). (C,D) Tracks of individual neural cell nuclei in wild-type and MZoep embryos at hindbrain level. Midline is marked with arrowhead. (E) Trajectory plots of cells in wild-type and MZoep hindbrain primordia. (F) Directionality plots for cells in wild-type and MZoep neural plates. (G,H) Speed and persistence of neural cell movements are disrupted from 12 hpf onwards in MZoep embryos (linear speed µm/min: wild-type cells 0.87 versus MZoep cells 0.56, ***P <0.0001 Student’s t-test; persistence: wild-type cells 0.85 versus MZoep cells 0.63, ***P <0.0002 Student’s t-test). (I,J,K,L) The hindbrain marker krox20 shows that the anterior-posterior pattern in hindbrain region is only mildly disrupted despite abnormal cell movements in MZoep neural primordia. H2B-RFP, histone H2B/red fluorescent protein fusion; hpf, hours post fertilization; MZoep, maternal-zygotic one-eyed pinhead; wt, wild-type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tdgf1^tz257/tz257
Observed In:	neural keel neural plate cell motility neural plate morphogenesis
Stage Range:	Bud to 20-25 somites

Phenotype Detail

Acknowledgments

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