FIGURE

Fig. 6

ID

ZDB-FIG-140602-17

Publication

Zannino et al., 2014 - prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements

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Fig. 6

Loss of prdm12b has a modest effect on hindbrain, but not spinal, motor neurons. (A–Q) Expression of Isl1/2 (A–D, K, L), olig2 (E–H), olig2:egfp (I, J), Isl1/2 together with hoxb1a:egfp (M, N) or Alcama (O–Q) in wild-type (A, C, E, F, I, K, M, O), PTB MO-injected (B, D, G, H, J, L, N, Q), or control MO-injected (P) embryos at 24 hpf (A–H) or 50 hpf (I–Q). Embryos are shown in lateral (A, B, I, J) or dorsal (C, D, K–Q) view with anterior to the left, or in cross section (E–H) with dorsal to the top. All scale bars are 35 μm, except I, J are 100 μm and E, H are 50 μm. RB – Rohon-Beard cells, MN – motor neurons, and (n)=cranial nerves.

Expression Data

Genes:	alcama EGFP olig2
Antibody:	Ab2-isl
Fish:	WT WT + MO1-prdm12b um8Tg vu12Tg um8Tg + MO1-prdm12b vu12Tg + MO1-prdm12b
Knockdown Reagent:	MO1-prdm12b
Anatomical Terms:	abducens motor nucleus diencephalon facial nerve motor nucleus hindbrain motor nucleus of vagal nerve primary motor neuron Rohon-Beard neuron spinal cord central region spinal cord motor neuron trigeminal motor nucleus
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-prdm12b um8Tg + MO1-prdm12b vu12Tg + MO1-prdm12b
Knockdown Reagent:	MO1-prdm12b
Observed In:	abducens motor nucleus abducens motor nucleus motor neuron facial nerve motor nucleus motor neuron spinal cord spinal cord motor neuron spinal cord motor neuron cell fate specification
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.