Fig. 1

Time course of deltaC expression in heat-shocked Tg(hsp70l:dlc) transgenic embryos. (A-D) Embryos at 14-16hpf were heat-shocked at 38.5°C either once, for 15min, with recovery at 28.5°C (A) or repeatedly (B-D), with recovery at 23°C between heat-shocks. (A) Whole mounts stained by ISH with a probe for deltaC mRNA, at different times after the single heat-shock, along with wild-type controls either heat-shocked (wt hs) or not (wt). ne7 for each time point. (B) Scheme of the repetitive heat-shock treatment. (C,D) Samples were fixed for FISH with deltaC riboprobe before and after each heat-shock; fluorescence was measured by confocal microscopy as an average over the three boxed regions (30×30μm²) marked in panel Ci. (C) Specimens before and after the first (i,ii) and second (iii,iv) heat-shocks. (D) Measurements for embryos subjected to up to five heat-shocks. Values are means of n embryos fixed before and after the end of each heat-shock, where (n_before, n_after)=(9, 10), (4, 4), (7, 4), (5, 3) and (7, 7) for the successive heat-shocks. Error bars show s.e.m. Asterisks indicate statistical significance of the difference between measurements before and after the given heat shock: *Pd0.06; **P<0.001; one-tailed t-test.