FIGURE

Fig. 4

ID

ZDB-FIG-140512-22

Publication

Osborn et al., 2014 - Loss of FTO antagonises Wnt signaling and leads to developmental defects associated with ciliopathies

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Fig. 4

Ca²⁺/Wnt signaling is activated in Fto deficient cells and zebrafish.

(A) Wnt signaling phospho antibody microarray.Control (Fto^+/+) and Fto knockout (Fto^/) MEFs were treated with Wnt3a and changes in phosphorylation of Wnt signaling proteins analysed by an antibody microarray. P/N: Phospho-Antibody/Non-Phospho-Antibody ratio. For detailed calculations see Methods. (B) Total CamKII, phosphorylated (Thr305) CaMKII, and pan phosphorylated PKC (Thr497) were analysed in control (Fto^+/+) and Fto knockout (Fto^/) MEFs treated with Wnt3a conditioned medium (+) for 0, 10, 20 and 40 minutes. Brackets indicate PKC isoforms, asterisks show a non-specific band. (C) Phosphorylated CaMKII (Thr287) is upregulated in the pronephric ducts (PND) of fto morphant embryos compared to uninjected controls, as shown by immunofluorescence at 48 hpf. Scale bar: 50 μm.

Expression Data

Antibody:	Ab3-camk2
Fish:	AB/TL AB/TL + MO1-fto
Knockdown Reagent:	MO1-fto
Anatomical Term:	pronephric duct
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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