FIGURE

Fig. 5

ID

ZDB-FIG-140507-9

Publication

Fero et al., 2014 - Impaired embryonic motility in dusp27 mutants reveals a developmental defect in myofibril structure

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Fig. 5

Muscle integrity is impaired in y241 mutants. (A) α-bungarotoxin-conjugated Alexa-Fluor-555 staining of 2 dpf wild-type sibling (left) and y241 mutant (right) embryos, showing equatorially located neuromuscular junctions (brackets). Scale bar: 50 μm. (B) Birefringence in live 5 dpf wild-type sibling (top) and y241 mutant (bottom) larvae detected using polarized light. Mutants show reduced and patchy birefringence. (C) Dodt-gradient-contrast illumination of muscle in 3 dpf larvae after removal of skin. Internal muscle striations are visible in myofibers stretching between somite boundaries in siblings (top) but not in y241 mutants (bottom). (D) 10 μm toluidine-blue-stained sections through the tail of 3 dpf wild-type siblings (top) and y241 mutants (bottom). (E) Kaede expression in highly variegated 24 hpf y241; UAS:kaede sibling embryos (top panels) and mutants (bottom panels) with DAPI staining to reveal nuclear positions. Somite boundaries observed in bright-field images are indicated by dashed lines. Scale bar: 30 μm.

Expression Data

Genes:	chrna1 Kaede
Fish:	dusp27^y241Et/+; s1999tTg (TL) dusp27^{y241Et/y241Et}; s1999tTg (TL)
Anatomical Terms:	fast muscle cell skeletal muscle cell acetylcholine-gated channel complex skeletal muscle cell neuromuscular junction of skeletal muscle fiber
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dusp27^y241Et/+; s1999tTg (TL) dusp27^{y241Et/y241Et}; s1999tTg (TL)
Observed In:	fast muscle cell myotome myotome neuromuscular junction of skeletal muscle fiber myotome skeletal muscle cell skeletal muscle cell myofibril skeletal muscle cell neuromuscular junction of skeletal muscle fiber
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

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