FIGURE

Fig. 7

ID

ZDB-FIG-140507-11

Publication

Fero et al., 2014 - Impaired embryonic motility in dusp27 mutants reveals a developmental defect in myofibril structure

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Fig. 7

Fast muscle myofibrils are disrupted in y241 mutants. (A–E) Immunofluorescence in fast-twitch fibers in 48 hpf sibling and y241 mutant embryos. (A) F310 labeling of fast-twitch-fiber myosin shows disruption of thick filaments. Scale bar: 25 μm. Insets show rotated transverse view through the somite (merged view of DAPI in blue and F310 in red). (B) CH1 labeling of tropomyosin shows disruption of thin filaments. Scale bar: 20 μm. (C) α-actinin staining shows loss of Z-lines in fast-twitch fibers. Scale bar: 25 μm. (D) Anti-titin staining. Scale bar: 30 μm. (E) DHPR in fast-twitch fibers. Scale bar: 25 μm. (F) Transmission electron microscopy in myofibers of 3 dpf y241 siblings and mutants, showing Z-lines (arrows) and triads (arrowheads). Scale bars: 500 nm.

Expression Data

Antibodies:	Ab-F310 Ab1-actn Ab1-cacna1s Ab1-tpm Ab1-ttn
Fish:	dusp27^{y241Et/y241Et} (TL) dusp27^y241Et/+ (TL)
Anatomical Terms:	fast muscle cell fast muscle cell muscle thin filament tropomyosin fast muscle cell striated muscle myosin thick filament fast muscle cell voltage-gated calcium channel complex fast muscle cell Z disc
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dusp27^{y241Et/y241Et} (TL)
Observed In:	fast muscle cell sarcomere fast muscle cell skeletal myofibril assembly fast muscle cell striated muscle myosin thick filament fast muscle cell striated muscle thin filament fast muscle cell T-tubule fast muscle cell Z disc
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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