Fig. 5

Sulf1 is required for correct temporal establishment and rearrangement of ventral neural patterning. (A-F) Expression of olig2 (A,D), nkx2.2a (B,E) and foxa2 (C,F) in transverse sections at 16 hpf in ctrlMO-injected (A-C) and sulf1MO-injected (D-F) embryos. Injection of ctrlMO does not affect expression of olig2 (A), nkx2.2a (B) and foxa2 (C), but injection of sulf1MO prevents upregulation of nkx2.2a (E) and foxa2 (F) as well as the dorsal shift of the olig2+ domain (D). (G-L) Expression of olig2 (G,J) and nkx2.2a (H,I,K,L) at 24 hpf in embryos injected with ctrlMO (G), mismatch MO (MisMO, H), sulf1MO^ATG (K), sulf1MO^splice (L) and co-injected with sulf1MO^splice and sulf1 mRNA (I). (M,N) Expression of nkx2.2a at 24 hpf in wild-type (M) and sulf1^sa199-/- (N) embryos. (O-R) Expression of olig2 (O,Q) and nkx2.2a (P,R) at 36 hpf in embryos injected with ctrlMO (O,P) and sulf1MO (Q,R). Brackets indicate position of the olig2+ domain, arrows point to nkx2.2a+ cells and stars mark the MFP in all panels. Dashed lines in O-R indicate dorsal boundary of the nkx2.2a+ domain. Scale bar: 20 μm.