FIGURE

Fig. 8

ID

ZDB-FIG-140422-46

Publication

Holly et al., 2014 - Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Injection of low dose (1 pg) of sfrp5 mRNA causes an increase in dorsal marker gene expression and BMP signaling. We used in situ hybridization to assess dorsal markers bambia and tbx5a and the ventral marker vax2 at 15 hpf in embryos injected with 1 pg sfrp5 mRNA. Both tbx5a and bambia expression domains are expanded in sfrp5 mRNA injected embryos compared to wildtype (A–D) while the vax2 domain is significantly reduced (E–F). We measured BMP signaling using the Tg(BMPRE-AAV.Mlp:eGFP) transgenic strain. In situ hybridization for eGFP mRNA in 15 hpf embryos injected with 1 pg sfrp5 mRNA indicate an increase in BMP signaling compared to wildtype (G–H). Prevalence of change in expression for all probes was quantified and significance was assessed using Fisher′s Exact Test (J, ^NNN, p<0.0001). qRT-PCR results for tbx5a expression at 15 hpf in sfrp5 mRNA injected embryos indicate a 1.17 fold change in expression (I, ^NN, p<0.01, Student′s t-test).

Expression Data

Genes:	bambia EGFP tbx5a vax2
Fish:	WT mw29Tg
Anatomical Terms:	optic vesicle optic vesicle dorsal region optic vesicle ventral region
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 388(2), Holly, V.L., Widen, S.A., Famulski, J.K., and Waskiewicz, A.J., Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development, 192-204, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.