ZFIN Image: Holly et al., 2014, Fig. 8

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Fig. 8

Injection of low dose (1 pg) of sfrp5 mRNA causes an increase in dorsal marker gene expression and BMP signaling. We used in situ hybridization to assess dorsal markers bambia and tbx5a and the ventral marker vax2 at 15 hpf in embryos injected with 1 pg sfrp5 mRNA. Both tbx5a and bambia expression domains are expanded in sfrp5 mRNA injected embryos compared to wildtype (A–D) while the vax2 domain is significantly reduced (E–F). We measured BMP signaling using the Tg(BMPRE-AAV.Mlp:eGFP) transgenic strain. In situ hybridization for eGFP mRNA in 15 hpf embryos injected with 1 pg sfrp5 mRNA indicate an increase in BMP signaling compared to wildtype (G–H). Prevalence of change in expression for all probes was quantified and significance was assessed using Fisher′s Exact Test (J, ^NNN, p<0.0001). qRT-PCR results for tbx5a expression at 15 hpf in sfrp5 mRNA injected embryos indicate a 1.17 fold change in expression (I, ^NN, p<0.01, Student′s t-test).

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Acknowledgments

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Reprinted from Developmental Biology, 388(2), Holly, V.L., Widen, S.A., Famulski, J.K., and Waskiewicz, A.J., Sfrp1a and Sfrp5 function as positive regulators of Wnt and BMP signaling during early retinal development, 192-204, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.