FIGURE

Fig. 3

ID

ZDB-FIG-140417-19

Publication

Fischer et al., 2013 - Abcb4 acts as multixenobiotic transporter and active barrier against chemical uptake in zebrafish (Danio rerio) embryos

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Fig. 3

Fluorescence micrographs of 48 hpf zebrafish embryos that had been incubated with 0.5 μM rhodamine B or 1.0 µM calcein-am either in the presence of different ABC transporter inhibitors (A) or upon knock-down of Abcb4 or Abcb5 (B). ABC transporter inhibitor compounds were cyclosporin A (CsA), PSC833 and MK571. Morpholino oligonucelotides were used for knock-down of Abcb4 (Abcb4-SP-MO/Abcb4-ATG-MO) or Abcb5 (Abcb5-SP-MO). Exposure water of control embryos contained dye only (ctrl, ctrl-MO). The morpholino control (ctrl-MO) served to exclude unspecific morpholino effects on dye accumulation in the embryos. Brighter appearing embryos indicate accumulation of higher amounts of dye in the embryo tissue.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-abcb4 WT + MO2-abcb4
Conditions:	chemical treatment: cyclosporin A chemical treatment: MK 571
Knockdown Reagents:	MO1-abcb4 MO2-abcb4
Observed In:	efflux transmembrane transporter activity
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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