ZFIN Image: Fischer et al., 2013, Fig. 3

Image

Figure Caption

Fig. 3

Fluorescence micrographs of 48 hpf zebrafish embryos that had been incubated with 0.5 μM rhodamine B or 1.0 µM calcein-am either in the presence of different ABC transporter inhibitors (A) or upon knock-down of Abcb4 or Abcb5 (B). ABC transporter inhibitor compounds were cyclosporin A (CsA), PSC833 and MK571. Morpholino oligonucelotides were used for knock-down of Abcb4 (Abcb4-SP-MO/Abcb4-ATG-MO) or Abcb5 (Abcb5-SP-MO). Exposure water of control embryos contained dye only (ctrl, ctrl-MO). The morpholino control (ctrl-MO) served to exclude unspecific morpholino effects on dye accumulation in the embryos. Brighter appearing embryos indicate accumulation of higher amounts of dye in the embryo tissue.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.