FIGURE

Fig. S4

ID

ZDB-FIG-140414-5

Publication

Miyares et al., 2013 - Long-Chain Acyl-CoA Synthetase 4A Regulates Smad Activity and Dorsoventral Patterning in the Zebrafish Embryo

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Fig. S4

Acsl4a is epistatic to the Bmp receptor Alk8, related to Figure 3.
(A) Representative bright-field images of 24 hpf embryos injected with 500 fmol acsl4a MO (class C4), 50 fmol bmp2b MO (class C5), 20-40 pg constitutively active (CA) alk8 mRNA (class V3), a combination of acsl4a MO and alk8CA mRNA (class C4), or a combination of bmp2b MO and alk8CA mRNA (class V3). Lateral view, anterior to the left. (B) Percentages of phenotypic classes. Dorsalization: strong (C5) to weak (C1). Ventralization: strong (V4) to weak (V1). n=283-298 embryos, from 3 separate experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-bmp2b
Knockdown Reagent:	MO1-bmp2b
Observed In:	whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 27(6), Miyares, R.L., Stein, C., Renisch, B., Anderson, J.L., Hammerschmidt, M., and Farber, S.A., Long-Chain Acyl-CoA Synthetase 4A Regulates Smad Activity and Dorsoventral Patterning in the Zebrafish Embryo, 635-647, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell