FIGURE

Fig. 4

ID

ZDB-FIG-140411-17

Publication

Ping et al., 2014 - Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase

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Fig. 4

The WMM complex plays essential roles during zebrafish embryogenesis. (A) Embryos injected with individual (WTAP-, METTL3-) or combined (METTL3+WTAP) MOs. The double knockdown showed more severe morphological defects, compared to other groups, at 24 hpf. Red arrows mark head and eyes, while blue arrows mark brain ventricle. The curve of the notochord is labeled by the double dashed lines. (B) Expression of somite marker myod in the morphants at 24 hpf. myod expression was increased in WTAP-, METTL3-, or double morphants. (C) Increased apoptosis (TUNEL assay) was observed in embryos injected with individual or combined MOs. (D) Overexpression of full-length or N-terminal but not C-terminal zebrafish WTAP mRNA prevented apoptosis in zebrafish WTAP-MO embryos. Note that there is auto-fluorescence on the yolk. Anterior to the left and dorsal is up. Scale bar, 250 μm. Supportive data were included in Supplementary information, Figures S5F and S6.

Expression Data

Gene:	myod1
Fish:	WT WT + MO1-mettl3 WT + MO1-wtap WT + MO1-mettl3 + MO1-wtap
Knockdown Reagents:	MO1-mettl3 MO1-wtap
Anatomical Term:	somite
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mettl3 WT + MO1-wtap WT + MO1-mettl3 + MO1-wtap
Knockdown Reagents:	MO1-mettl3 MO1-wtap
Observed In:	somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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