FIGURE

Fig. S8

ID

ZDB-FIG-140409-26

Publication

Eroglu et al., 2014 - An essential role for heat shock transcription factor binding protein 1 (HSBP1) during early embryonic development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S8

HSBP1 morpholino-injected embryos show aberrant expression of genes involved in neurodevelopment.

In situ hybridization analyses showing the expression of the indicated markers in control MO-injected or HSBP1 MO-injected embryos at the indicated times post fertilization. The markers were as follows: Anf (a-b), Eng2 (c-h), Krox-20 (i-n), Ntl (o-r), and Hlx1 (s-t). Morphant phenotypes were more than 90%. n=40. Embryos are dorsal view (a-I; o-p) or lateral view (m-n; q-t). For in situ hybridization analyses a total of 3000 embryos were injected and 200 embryos were used per probe. No nonspecific hybridization was observed with sense probes (data not shown). Embryos were photographed using a SPOT camera mounted on a dissecting microscope. Mag. 40x.

Expression Data

Genes:	egr2b en2a hesx1 hlx1 tbxta
Fish:	WT WT + MO1-hsbp1b
Knockdown Reagent:	MO1-hsbp1b
Anatomical Terms:	forebrain forebrain neural plate hindbrain midbrain midbrain hindbrain boundary midbrain hindbrain boundary neural keel midbrain hindbrain boundary neural plate notochord presumptive rhombomere 3 presumptive rhombomere 5 rhombomere 3 rhombomere 5
Stage Range:	Bud to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-hsbp1b
Knockdown Reagent:	MO1-hsbp1b
Observed In:	central nervous system central nervous system development forebrain forebrain neural plate hindbrain midbrain midbrain hindbrain boundary midbrain hindbrain boundary neural keel midbrain hindbrain boundary neural plate notochord presumptive rhombomere 3 presumptive rhombomere 5 rhombomere 3 rhombomere 5
Stage Range:	Bud to Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 386(2), Eroglu, B., Min, J.N., Zhang, Y., Szurek, E., Moskophidis, D., Eroglu, A., and Mivechi, N.F., An essential role for heat shock transcription factor binding protein 1 (HSBP1) during early embryonic development, 448-460, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.