FIGURE

Fig. 2

ID

ZDB-FIG-140403-17

Publication

George et al., 2014 - Synaptojanin 1 is required for endolysosomal trafficking of synaptic proteins in cone photoreceptor inner segments

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Fig. 2

Some synaptic proteins are mislocalized in nrc^a14 cone photoreceptors.

Tg(TαCP:spH) WT and nrc^a14 5 dpf retinal slices were stained with an antibody against the ribbon synapse protein RibeyeB (A–F). In WT photoreceptors, RibeyeB was found only at synaptic terminals (A). In nrc^a14 cone photoreceptors, RibeyeB (D, arrowhead) and VAMP2 (E, arrowhead) were detected in both synaptic terminals and ISs. Mislocalized signals for RibeyeB (magenta) and VAMP2 (green) were coincident in the ISs of nrc^a14 cone photoreceptors (F, arrowhead). In contrast, the synaptic vesicle protein Synaptophysin (Syp-CFP) had a primarily synaptic distribution in both WT (G) and nrc^a14 (H) cone photoreceptors. Live confocal images were taken of 5 dpf Tg(TαCP:Syp-CFP) WT and nrc^a14 larvae. Syn = photoreceptor synapses, IS = inner segment. Scale bar = 2 μm.

Expression Data

Genes:	CFP ctbp2l spHGFP
Antibody:	Ab1-ctbp2l
Fish:	w63Tg w139Tg synj1^a14/a14; w63Tg synj1^a14/a14; w139Tg
Anatomical Terms:	retinal cone cell photoreceptor inner segment retinal cone cell presynaptic active zone
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	synj1^a14/a14; w63Tg
Observed In:	retinal cone cell photoreceptor inner segment
Stage:	Day 5

Phenotype Detail

Acknowledgments

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