FIGURE

Fig. 2

ID

ZDB-FIG-140328-17

Publication

Lister et al., 2014 - A Conditional Zebrafish MITF Mutation Reveals MITF Levels Are Critical for Melanoma Promotion vs. Regression In Vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Comparative histopathology of BRAF^V600E melanomas. (a) Cross-section of adult BRAF^V600Emitf zebrafish with superficial spreading melanoma (dotted line). Infiltrating melanophages in the kidney are indicated (yellow arrows). i, intestine; k, kidney; l, liver; m, muscle; o, ovary; s, spinal column; sb, swimbladder. (Top and bottom panels) Hematoxylin and eosin (H&E) stain of BRAF^V600Emitf melanoma, indicating large melanophages (red arrows), spindle or epithelioid cell shapes (yellow arrows), and pigmented melanoma cells (white arrow). Scale bars=20 μm. (b) Cross-section of adult BRAF^V600Ep53 zebrafish with invasive melanoma (dotted line). (Top and bottom panels) H&E stain of BRAF^V600Ep53 melanoma, indicating pigmented melanoma cells (white arrows) and nuclear pleomorphisms (yellow arrow). Scale bars=20 μm. (c–f) Immunohistochemistry staining for (c) phospho-extracellular signal–regulated kinase (ERK), (d) p53, (e) Melan-A, and (f) phospho-histone H3. Scale bars=50 μm. (g). Box plot of mean percentage phospho-histone H3–stained cells in BRAF^V600Emitf and BRAF^V600Ep53 tumors (n=11 melanomas of each genotype). Bars represent interquartile range; Student’s t-test P=0.0078.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^vc7/vc7; czt13Tg/czt13Tg tp53^zdf1/zdf1; czt13Tg
Observed In:	whole organism melanoma
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Invest. Dermatol.