FIGURE

Fig. 1

ID

ZDB-FIG-140328-16

Publication

Lister et al., 2014 - A Conditional Zebrafish MITF Mutation Reveals MITF Levels Are Critical for Melanoma Promotion vs. Regression In Vivo

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Fig. 1

The microphthalmia-associated transcription factor (MITF) is oncogenic with BRAF^V600E in melanomagenesis. (a) Adult wild-type zebrafish or (b) adult mitfa^vc7 mutant zebrafish living in water at 28 °C or (c, d) <26 °C. At <26 °C some melanocytes are visible in the body (d: enlarged region, white arrows). (e) Adult transgenic line expressing human BRAF^V600E in the melanocytes. (f–i) Genetic crosses of BRAF^V600Emitf at the semirestrictive temperatures develop nevi (*) and melanoma (on the tail of the middle fish, and on the head of the bottom fish). (j) Melanoma incidence curves of BRAF^V600Ep53 and BRAF^V600Emitf (<26 °C) genetic crosses. (k) Real-time PCR (RT-PCR) analysis of the mitfa transcript in BRAF^V600Ep53 and BRAF^V600Emitf melanomas.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^vc7/vc7 mitfa^vc7/vc7; czt13Tg/czt13Tg
Conditions:	cold shock heat shock
Observed In:	head melanoma melanocyte post-vent region melanoma post-vent region naevus whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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