FIGURE

Fig. 5

ID

ZDB-FIG-140326-26

Publication

Yan et al., 2002 - A zebrafish sox9 gene required for cartilage morphogenesis

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Fig. 5

Morpholino antisense oligonucleotides directed against sox9a inhibit splicing and transport of sox9a transcript. (A) RNase protection assays of sox9a transcript in 28 hpf wild-type animals injected at the one-cell stage with 2 nl of different concentrations of MO, normalized against odc. (B) RNase protection assays of sox9a transcript in animals 28 hpf or 4 dpf either treated (+) or not treated (–) with 5.0 ng MO, normalized against odc. Graphs show the amount of RNA present as a percent of untreated controls. (C) Transcript for sox9a accumulates in the cytoplasm in wild type, but in the nucleus in splice-directed MO injected embryos at 28 hpf. In jef^tw37 embryos, transcript is visible both in the cytoplasm and in the nucleus. Dorsal views, anterior is upwards. Equal amounts of MO directed against the donor sites for intron 1 and 2. Scale bars: 100 μm.

Expression Data

Gene:	sox9a
Fish:	WT sox9a^tw37/tw37 WT + MO2-sox9a + MO3-sox9a
Knockdown Reagents:	MO2-sox9a MO3-sox9a
Anatomical Terms:	neurogenic placode cytoplasm neurogenic placode nucleus
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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