Lateral views of whole mount embryos immunostained for expression of acetylated α-tubulin and anti-HuC in neural crest derivatives at 24-72 hpf. Pattern of DRG, lateral line pathfinding, trigeminal ganglia formation in wild-type (A,C, trunk; E,G, head) and nrd mutant embryos (B,D, trunk; F,K, head). nrd embryos have a reduction in the number of neurons per ganglion in the DRG at 72 hpf (B; see also Table 1) and the neuromasts (not shown) (A′,B′) High magnification images of the ganglion (arrows in A and B) to show the reduction in the number of cells per ganglion. (C,D) The lateral line (LL) pathfinding is misaligned (arrow) at 72 hpf. Within the trigeminal ganglia (TG), cells derive from the neural crest as well as ectodermal placode. At 24 hpf (E,F), mutant embryos (F) show a reduction in the dorsal ganglia corresponding to the neural crest derived portion (arrows). But by 48 hpf (G,H), the trigeminal ganglia in the mutant (H) is close to the normal size, suggesting that the placodal or late migrating neural crest population may be compensating for the loss of the neural crest. DRG, dorsal root ganglia.
|
