FIGURE

Fig. 3

ID

ZDB-FIG-140213-3

Publication

Wu et al., 2014 - Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development

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Fig. 3

The med12/kgt mutant shows defects in habenular development. The habenular differentiation markers, kctd12.1, kctd12.2, nrp1a, and cadps2 were all absent in med12 mutants at 4 dpf (A-D′) and 2 dpf (G, G′), while the habenular cells were still present in the dorsal diencephalon in med12 mutants as shown by ToPro3 nuclear staining at 4 dpf (E, E′; habenular nuclei were outlined in white; the dark shadow on the left habenula in E′ is due to an overlying pigment cell). Fewer Elavl3-expressing neurons were observed (F, F′; at 2 dpf) and the expression of cxcr4b was significantly reduced in med12 mutants when compared to WT (H, H′; at 36 hpf). In med12 mutants, the presumptive habenular progenitor marker, dbx1b, was absent early (lateral views, at 28 hpf; I, I′) but reappeared in later stages (at 48 hpf, J, J′).

Expression Data

Genes:	cadps2 cxcr4b dbx1b GFP kctd12.1 kctd12.2 nrp1a
Antibody:	Ab1-elavl
Fish:	WT med12^vu304/vu304 zf104Tg med12^vu304/vu304; zf104Tg
Anatomical Terms:	habenula pineal complex pineal complex neuron
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	med12^vu304/vu304
Observed In:	habenula development
Stage Range:	Prim-25 to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 385(1), Wu, S.Y., de Borsetti, N.H., Bain, E.J., Bulow, C.R., and Gamse, J.T., Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development, 13-22, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.