FIGURE

Fig. 4

ID

ZDB-FIG-131223-7

Publication

Choorapoikayil et al., 2012 - Haploinsufficiency of tumor suppressor pten predisposes to hemangiosarcoma in zebrafish

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Fig. 4

Elevated Akt/PKB signaling in Pten mutant tumors. Immunohistochemistry on transversal sections from a ptena^+/-ptenb^-/- mutant fish, using pAkt- and pGSK-3β-specific antibodies. (A) Tumor area stains weakly positive for pAkt, whereas (B) control vessel (arrow) from the same section is not stained (n=10). (C) Tumor area is highly positive for pGSK-3β, whereas (D) cells from a control vessel (arrow) from the same section only stain mildly positive (n=6). (E) The cranial part of a ptena^+/-ptenb^-/- mutant that developed a tumor was dissected into two fragments, one harboring tumor tissue (tumor) and the other representing control tissue (control). The samples were lysed and the lysates were run on a denaturing SDS-polyacrylamide gel. The proteins were transferred to a PVDF membrane and after blocking the blot was probed with anti-pAkt antibody, stripped and sequentially probed with anti-Akt, anti-pGSK-3, anti-PTEN and, as a loading control, anti-actin.

Expression Data

Antibodies:	Ab2-gsk3b Ab8-akt
Fish:	ptena^hu1864/+; ptenb^{hu1435/hu1435}
Anatomical Terms:	endothelial cell eye hemangiosarcoma
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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