FIGURE

Fig. 7

ID

ZDB-FIG-131220-41

Publication

Angonin et al., 2013 - Nkd1 functions as a passive antagonist of wnt signaling

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Fig. 7

Figure 7. Trilobite (vangl2) mutants are sensitive to Nkd1.

Knockdown of Nkd1 with morpholinos (D, E) or overexpression of Nkd1 (I, J) does not have an obvious effect during early somitogenesis. However, at 1 dpf, knockdown or overexpression of Nkd1 in tri mutants results in an increase in cyclopia (K, L, O). Before the onset of gastrulation, at 50% epiboly, embryos generated from a tri+/- X tri+/- parental cross are sensitive to Nkd1 overexpression, demonstrated by a reduction or absence of gsc expression (M; n=32, N; 56% of embryos with reduced expression, n=25). (O) The cyclopic index was calculated using criteria established in Marlow et al., 1998 [43]. n values reflect the number of tri-/- embryos. Error bars represent standard error.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vangl2^m747/m747 (AB) vangl2^m747/m747 + MO2-nkd1 (AB)
Knockdown Reagent:	MO2-nkd1
Observed In:	convergent extension
Stage Range:	50%-epiboly to Prim-5

Phenotype Detail

Acknowledgments

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