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Fig. 7
Figure 7. Trilobite (vangl2) mutants are sensitive to Nkd1.
Knockdown of Nkd1 with morpholinos (D, E) or overexpression of Nkd1 (I, J) does not have an obvious effect during early somitogenesis. However, at 1 dpf, knockdown or overexpression of Nkd1 in tri mutants results in an increase in cyclopia (K, L, O). Before the onset of gastrulation, at 50% epiboly, embryos generated from a tri+/- X tri+/- parental cross are sensitive to Nkd1 overexpression, demonstrated by a reduction or absence of gsc expression (M; n=32, N; 56% of embryos with reduced expression, n=25). (O) The cyclopic index was calculated using criteria established in Marlow et al., 1998 [43]. n values reflect the number of tri-/- embryos. Error bars represent standard error.