spt mutants possess more nephric precursors, but have fewer endothelial and hematopoietic precursors. (A–G) Expression in the trunk at 14.5 h of: (A) pax2a and (B) jag2, in nephric cells; (C) fli1a and (D) cdh5, in endothelial cells; and (E) spi1, in white, (F) gata1, in red, and (G) tal1, in all blood cells (arrow designates a single cell). (H, I) Expression in the head at 14.5 h of: (H) fli1a, in endothelial cells; and (I) spi1, in macrophages. (J, K) Quantification of intermediate mesoderm precursors for: (J) trunk, and (K) head. Graphs show the average number of cells per marker including standard error; 9 embryos for each category were counted. Embryos are shown from a (A–G) posterior view, or (H, I) anterior view with dorsal to the top.
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