FIGURE

Fig. 5

ID

ZDB-FIG-131018-1

Publication

Matsui et al., 2012 - Celf1 regulation of dmrt2a is required for somite symmetry and left-right patterning during zebrafish development

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Fig. 5

Celf1 promotes dmrt2a mRNA decay in vivo. (A) Effect of Celf1 on dmrt2a expression in zebrafish embryos. Total RNAs extracted from embryos injected with control-MO, celf1-MOs, dmrt2a-TP-MO, mRFP mRNA, celf1 mRNA or celf1^Δ^linker mRNA at 12-14 hpf were subjected to qPCR for dmrt2a and β-actin. The samples were normalized to β-actin as a reference. (B) dmrt2a mRNA is stabilized in embryos injected with celf1-MOs or dmrt2a-TP-MO. Decay rate of dmrt2a mRNA was assessed in embryos injected with control-MO, celf1-MOs or dmrt2a-TP-MO by qPCR following transcription inhibition using Actinomycin D. The samples were normalized to β-actin as a reference. control-MO: t_1/2=317±11 minutes; celf1-MOs: t_1/2=835±3 minutes; dmrt2a-TP-MO: t_1/2=1487±4 minutes. Error bars represent s.d.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-celf1 + MO2-celf1 WT + MO3-dmrt2a
Knockdown Reagents:	MO1-celf1 MO2-celf1 MO3-dmrt2a
Observed In:	mRNA catabolic process
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

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