FIGURE

Fig. S6

ID

ZDB-FIG-131015-58

Publication

Wei et al., 2013 - miR-153 Regulates SNAP-25, Synaptic Transmission, and Neuronal Development

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Fig. S6

miR-153 regulates secondary motor neuron development. (A) Immunofluorescence was performed on whole mount zebrafish embryos at 55 hpf using Zn-8 antibodies to label secondary motor neurons. Confocal images were acquired from the same somites for all embryos, as indicated. (B) miR-153 knockdown (miR-153^MO) and snap-25a,b overexpression significantly increased the growth of secondary motor neuron axons (arrows). Overexpression of miR-153 or knockdown of snap-25a,b (snap-25a,b^MO) caused severe defects in axon development and architecture (asterisks). Scale bar: 40 μm.

Expression Data

Antibody:	zn-8
Fish:	WT WT + MO1-dre-mir-153a + MO1-mir153 WT + MO1-snap25 + MO1-snap25a WT + MO1-dre-mir-153a + MO1-mir153 + MO1-snap25 + MO1-snap25a
Knockdown Reagents:	MO1-dre-mir-153a MO1-mir153 MO1-snap25 MO1-snap25a
Anatomical Term:	spinal cord secondary motor neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dre-mir-153a + MO1-mir153 WT + MO1-snap25 + MO1-snap25a
Knockdown Reagents:	MO1-dre-mir-153a MO1-mir153 MO1-snap25 MO1-snap25a
Observed In:	secondary motor neuron axon secondary motor neuron axon extension
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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