Fig. S3

Genes with stochastic early expression. Genes belonging to this class show stochastic expression at the onset of the zygotic transcription. We could detect clusters of cells both in wild type and ich embryos that upregulated these particular genes, but no clear pattern emerged. For aplnrb (A–F) and isg15 (G–N), the expression pattern later became more coherent, and localized around the germring. Interestingly, although no clear expression pattern emerged, foxo3b expression in general appeared higher in wild type embryos (O–V). Later stages of foxo3b expression in wild type embryos also showed stochastic and varying expression patterns (A′–H′). All sphere and 30% epiboly stage embryos are shown from an animal view. Shield, 70% epiboly and bud stage embryos are shown from a lateral view, with dorsal to the right.