Fig. 4

Expression patterns of newly characterized genes.

(A) ier2 expression starts early in the future dorsal side, persists until early mid-gastrulation, then fades away, and becomes detectable only during somitogenesis in a stripe in the hindbrain area, and in the tailbud. (B) rnd1l expression is weakly upregulated in all the blastomeres after MBT, and it is expressed at higher levels in a few dorsal precursors. Later the expression becomes restricted in the mesoderm (both axial and non-axial), where it is progressively downregulated. During somitogenesis two prominent neural expression domains appear: in the eyefield and in the hindbrain. (C) ppp1r3ca expression starts at the dorsal edge of the embryo, and later it is upregulated in a narrow stripe around the germring. Expression weakens during late gastrula stages, but it will be upregulated during somitogenesis in the anterior forebrain and in the posterior tailbud regions. (D) LOC100334443 expression is strongly upregulated after the onset of zygotic transcription. During gastrulation it becomes restricted to the ectoderm, where it is gradually downregulated, except in the dorsal forerunner cells. During somitogenesis expression is also prominent in the neurectoderm, especially in the hindbrain. All embryos are shown from lateral view, dorsal to the right.