FIGURE

Fig. 3

ID

ZDB-FIG-130826-56

Publication

Pascoal et al., 2013 - Notch signalling is required for the formation of structurally stable muscle fibres in zebrafish

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Fig. 3

Cartilage and muscle masses are specified in mib^ta52b mutants.

Whole-mount in situ hybridization of 68 hpf siblings (n = 15) (A, A′), (n = 12) (C, C′), (n = 14) (E, E′) and mib^ta52b mutants (n = 12) (B, B′), (n = 15) (D, D′), (n = 11) (F, F′) using sox9a and sox9b as cartilage markers and myod as a muscle marker, showing a slight upregulation of these genes in mib^ta52b mutant pectoral fin. (A-F) Whole larvae. (A′-F′) Detached pectoral fins with distal to the right. Double fluorescent in situ hybridization on sections using sox9b (green) and myod (red), counterstained with DAPI to label the nuclei (blue) shows no signs of cell mixing in a sibling (n = 7) (G) and a mib^ta52b mutant pectoral fins (n = 9) (H). An upregulation of these genes is observed in mib^ta52b mutant pectoral fins when compared with the sibling.

Expression Data

Genes:	myod1 sox9a sox9b
Fish:	WT mib1^ta52b/ta52b
Anatomical Terms:	pectoral fin cartilage pectoral fin musculature
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mib1^ta52b/ta52b
Observed In:	pectoral fin cartilage pectoral fin musculature
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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