FIGURE

Fig. 5

ID

ZDB-FIG-130815-9

Publication

Gupta et al., 2013 - Loss of Catalytically Inactive Lipid Phosphatase Myotubularin-related Protein 12 Impairs Myotubularin Stability and Promotes Centronuclear Myopathy in Zebrafish

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Fig. 5

Myotubularin and PtdIns3P alterations in mtmr12 morphants.

(A) Immunofluorescence of control and mtmr12 knockdown fish showed significantly decreased myotubularin staining in mtmr12 knockdown fish in images taken under identical conditions. Immunofluorescence detection of PtdIns3P showed apparent increases of this myotubularin substrate in mtmr12 morphant embryos as compared to controls. (B) PtdIns3P levels are increased in mtmr12, mtm1 and mtm1-mtmr12 morphant zebrafish, *Pd0.05. Total lipids were extracted from zebrafish at 3 dpf and PtdIns3P levels were measured using a lipid-protein overlay enzyme-linked immunosorbent assay.

Expression Data

Antibodies:	Ab1-mtm1 Ab1-ptdIns(3,4,5)P3
Fish:	AB AB + MO2-mtmr12
Knockdown Reagent:	MO2-mtmr12
Anatomical Term:	skeletal muscle T-tubule
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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