FIGURE

Fig. 4

ID

ZDB-FIG-130815-8

Publication

Gupta et al., 2013 - Loss of Catalytically Inactive Lipid Phosphatase Myotubularin-related Protein 12 Impairs Myotubularin Stability and Promotes Centronuclear Myopathy in Zebrafish

Other Figures

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Fig. 4

Ultrastructural abnormalities in MTMR12 deficiency.

Transmission electron micrographs of skeletal muscles in control and morphant fish. In comparison to wild-type controls (A), mtmr12 morphant fish displayed abnormal triads (C, high magnification, inset). mtm1 morphant muscle also had absent or disorganized triads (B). mtmr12-mtm1 double knockdown morphants displayed exacerbated defects in muscle with many absent Z-lines in addition to disorganized triads (D inset shows high magnification view). Higher magnification examination showed whorled membranous structures in mtmr12 and mtmr12-mtm1 morphants (E–F). (G) Histograms represent quantification of disorganized triads of the single or double morphants. Total number of triads were counted in at least 15 myofibers within each embryo (n = 5 embryos). *Pd0.05, ns: statistically not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-mtmr12 AB + MO4-mtm1 AB + MO2-mtmr12 + MO4-mtm1
Knockdown Reagents:	MO2-mtmr12 MO4-mtm1
Observed In:	skeletal muscle skeletal muscle T-tubule skeletal muscle Z disc
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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