FIGURE

Fig. 6

ID

ZDB-FIG-130725-18

Publication

Helker et al., 2013 - The zebrafish common cardinal veins develop by a novel mechanism: lumen ensheathment

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Fig. 6

Cdh5 is required for delamination and lumen extension in the CCV. (A-D,F,G) Confocal projections of the CCV at 32 hpf (A-D) and 36 hpf (F,G) of Tg(kdrl:EGFP)^s843 (C,D,F,G) or Tg(fli1a:EGFP)^y1 (A,B) embryos. (A-D′) ECs of cdh5^ubs8 mutant embryos (B,B′) fail to delaminate and do not align around the lumen. Lack of blood flow (tnnt2a MO, D,D2) does not influence lumen formation. (A2-D2) Transverse sections of A-D, showing the lumen (asterisk) imaged at the level of the dashed line in A-D. (E) Quantification of the length of the CCV. (F-H) Directionality of collective migration is impaired in cdh5^ubs8 mutant embryos (G, white lines), whereas migration track length or speed was unaffected (H). Single ECs (red dots) were tracked for 4 hours. ^**P<0.01, ^***P<0.001; n.s., not significant; error bars indicate s.e.m.

Expression Data

Gene:	EGFP
Fish:	s843Tg y1Tg s843Tg + MO1-tnnt2a cdh5^ubs8/ubs8; s843Tg cdh5^ubs8/ubs8; y1Tg
Knockdown Reagent:	MO1-tnnt2a
Anatomical Term:	common cardinal vein blood vessel endothelial cell
Stage Range:	Prim-15 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdh5^ubs8/ubs8; s843Tg
Observed In:	blood vessel endothelial cell delamination involved in blood vessel lumen ensheathment common cardinal vein common cardinal vein blood vessel endothelial cell migration endothelial tube lumen extension involved in blood vessel lumen ensheathment
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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