FIGURE

Fig. S4

ID

ZDB-FIG-130516-50

Publication

Espín et al., 2013 - TNF receptors regulate vascular homeostasis through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Validation and effects of two additional MOs targeting zebrafish TNFRSF1B. (A) RT-PCR analysis of TNFRSF1B-E1/I1-mo induced altered splicing of the tnfrsf1b transcript at 3 dpf. A dose-dependent decline of the wild type RNA was observed, suggesting the insertion of intron 1, which has 15,726 bp length. This insertion resulted in a predicted TNFRSF1B protein lacking both TNFα binding and signaling domains. Samples without template (-) gave no amplifications. The annealing of MO (dashed line) and the inframe premature stop codons (arrowheads) are indicated. (B) Embryos injected with 8 ng/egg (1 mM) TNFRSF1B-E1/I1-mo (middle panel) or TNFRSF1B-ATG-mo showed impaired differentiation of the caudal artery (CA) and caudal vein (CV) (asterisks), hemorrhages throughout the body (arrowheads) and altered blood circulation. A control larvae injected with STD-mo is shown on the left panel for comparison. Scale bars, 100 μm. (C) The vascular defects shown in (B) were scored at 72 hpf as indicated in the legend to Figure 2. ***P<0.0001 according to Chi-square contingency test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sd2Tg; y1Tg + MO3-tnfrsf1b sd2Tg; y1Tg + MO4-tnfrsf1b
Knockdown Reagents:	MO3-tnfrsf1b MO4-tnfrsf1b
Observed In:	blood circulation caudal artery caudal vein whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.