FIGURE

Fig. 4

ID

ZDB-FIG-130503-16

Publication

Amali et al., 2013 - Zebrafish hoxd4a Acts Upstream of meis1.1 to Direct Vasculogenesis, Angiogenesis and Hematopoiesis

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Fig. 4

Loss of hoxd4a function impairs development of the vasculature.

(A–D) Fluorescent images of the trunk and tail regions of Tg(fli1:EGFP) embryos at 48 hpf. The panels present merged bright field and fluorescent images (A,C) or fluorescent images only (B,D) The normal pattern of the vasculature (A,B) is severely disrupted in hoxd4a morphants (C,D) Dorsal extremities of ISV sprouts that fail to contact the DLAV are marked by white dots (D). The caudal vein plexus of control embryos (E, arrowheads) is replaced by a disorganized mass of endothelial tissue in hoxd4a morphants (F, arrowheads). (G–I) Alkaline phosphatase staining at 72 hpf revealing the vasculature in (G) control-injected larvae, (H) hoxd4a morphants, and (I) rescued larvae co-injected with capped mRNA for hoxd4a. Dorsal aorta (DA), posterior cardinal vein (PCV), inter-segmental vessels (ISV), caudal artery (CA), dorsal longitudinal anastomotic vessel (DLAV), caudal vein (CV) and vertebral artery (VTA). All images show lateral views, with anterior to the left and dorsal on top. Scale bars equal 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-hoxd4a y1Tg + MO1-hoxd4a
Knockdown Reagent:	MO1-hoxd4a
Observed In:	angiogenesis caudal vein plexus intersegmental vessel subintestinal vein vasculature vasculogenesis vertebral artery
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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