FIGURE

Fig. 5

ID

ZDB-FIG-130412-20

Publication

Hsieh et al., 2013 - Dner inhibits neural progenitor proliferation and induces neuronal and glial differentiation in zebrafish

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Fig. 5

Dner inhibits the expression of glial precursors and induces the formation of mature glial cells. (A) Expression of slc1a3a was reduced in embryos that overexpressed dner or id-dner at the 18-somite stage. Injections with either MO1 or MO2 upregulated the expression of slc1a3a, and this effect could be rescued by concomitant injection with dner cRNA. (B) Overexpression of dner or id-dner induced the expression of mag at 3 dpf. In contrast, mag expression was significantly downregulated in the morpholino-injected embryos, and this effect was rescued by co-injection with dner cRNA. (C) qPCR analysis confirmed the results of the in situ hybridization shown in A–B. ^NN, p<0.01; ^NNN, p<0.001.

Expression Data

Genes:	mag slc1a3a
Fish:	TU TU + MO1-dner TU + MO2-dner
Knockdown Reagents:	MO1-dner MO2-dner
Anatomical Terms:	glial cell (sensu Vertebrata) glioblast (sensu Vertebrata)
Stage Range:	14-19 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 375(1), Hsieh, F.Y., Ma, T.L., Shih, H.Y., Lin, S.J., Huang, C.W., Wang, H.Y., and Cheng, Y.C., Dner inhibits neural progenitor proliferation and induces neuronal and glial differentiation in zebrafish, 1-12, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.