FIGURE

Fig. 4

ID

ZDB-FIG-130326-65

Publication

Zhang et al., 2013 - The Role of egr1 in Early Zebrafish Retinogenesis

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Fig. 4

Immunohistochemical analysis of the INL cells in the Egr1-morphant retinas.

Immunohistochemical analysis of the INL cells in the controls (5misCTLMO) and Egr1 morphants (egr1sMO) was performed with several cell markers at 72 and 120 hpf. These include anti-5E11 (5E11; A-D), anti-parvalbumin (Parv; E-H), anti-GABA (GABA; I-L) and anti-Islet1 (Islet1; M-P) for ACs; anti-PKC²1 (PKC; Q-T) for BCs; anti-GS (GS; U-X) for MCs; and Islet1 and anti-Prox1 (Prox1; Y-AB) for HCs. In short, the analysis has revealed that Egr1 knockdown specifically compromised the differentiation of Parv+ and GABA+ ACs. See text, Table 1 and 2 for further discussion and additional results for the specific effects on HCs differentiation in Figure 6. For all sections, the lens is on the left and dorsal is up. Scale bar = 50 µm.

Expression Data

Antibodies:	Ab-5E11 Ab1-glul Ab1-prkcb Ab1-pvalb Ab2-GABA Ab2-isl Ab8-prox1
Fish:	AB AB + MO6-egr1
Knockdown Reagent:	MO6-egr1
Anatomical Terms:	amacrine cell Muller cell retinal bipolar neuron retinal inner nuclear layer horizontal cell
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO6-egr1
Knockdown Reagent:	MO6-egr1
Observed In:	amacrine cell amacrine cell differentiation Muller cell neural retina development retinal bipolar neuron
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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