Fig. 4

Enc1l expression is controlled by RA, FGF, and Wnt signaling in the KV of zebrafish embryos. The embryos were stained at the bud stage. (A): In control embryos, enc1l is expressed in the KV. (B, C) In fgfr1-MO injected or SU5402-treated embryos, the expression of enc1l in KV is reduced compared to control. (D): The enc1l expression level in KV is increased by over-expression of fgf8-mRNA. (E): The enc1l expression level is reduced in RA-treated embryos. (F): The enc1l expression level is increased by DEAB treatment. (G): Injection of wnt8-MO reduces enc1l expression in the KV. (H): Treatment with LiCl, which increases canonical Wnt signaling, increases enc1l expression level. (I): Overexpression of wnt8-mRNA increases enc1l expression. (J): Injection of ntl-MO can reduce the enc1l expression level. Up- (L) and down- (K) regulation of FGF signaling has no effect on RA-treated embryos; however, in DEAB-treated embryos, the down-regulation of FGF signaling (M) can restore the enc1l expression level, though the up-regulation of FGF signaling (N) has no affect. In DEAB-treated embryos, injection of wnt8-MO (O) or wnt8-mRNA (P) restores enc1l expression. The number of the representative phenotype embryos versus the number of total analyzed embryosis shown at the top of the right-hand corner of the picture.