Fig. 2

csrnp1a knockdown decreases the number of blood cells in circulation.

O-Dianisidine stain labels erythrocytes (A, B, C, D, E) and Diaminobenzidine (DAB) blood cells in general at the ICM (F and G). The injection of csrnp1a morpholino produces a dramatic reduction of blood cells in 34% of the injected embryos (n = 16/47) at 33 hpf (A, B, C, F, G) which is reverted at 48 hpf (D, E) (all are lateral views, anterior to the left). (H) Chart summarizing the morpholino dose-phenotype quantifications of 26 hpf control embryos (n = 71) and injected with 2,5 ng (n = 69), 5 ng (n = 85) or 10 ng (n = 87) of Mocsrnp1a spt morpholino. (I) qPCR with primers located in exon 2 and 3 of 24 hpf control embryos (C) and splicing morphants injected with two concentrations, 2,5 and 5 ng per embryo, showing the relative expression of csrnp1a message, which decreases significantly in a dose-dependent manner (t student p<0,01).