Fig. S4

csrnp1a morphants exhibit an increase in cell death due to morpholino toxicity which is not responsible for the reduction in blood cells in circulation. We analyzed cell death by acridine orange in embryos injected with control morpholino (A), csrnp1a morpholino (C), p53 morpholino (E), or a mixture of csrnp1a and p53 morpholinos (G). A clear increased in cell death is detected in csrnp1a morphant embryos (86,7%; n = 58/67), which is reversed by p53 co-injection (16,7%; n = 16/96). The cell blood phenotype was screened at 33 hpf using O-Dianisidine stain in (B) control, (D) csrnp1a, (F) p53 morphant embryos and in embryos co-injected with both morpholinos (H). The csrnp1a, p53 co-injected embryos (H; 39,6% n = 21/53) have the same penetrance of the blood phenotype as csrnp1a morphants (F; 35,7% n = 15/42) (red arrows). It worth mention that the slight head reduction exhibited by csrnp1a morphant embryos was also detected in co-injected embryos (black arrows). All are lateral views, anterior to the left.