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Fig. 2

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ZDB-FIG-130208-5

Publication

Kizil et al., 2012 - Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3

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Fig. 2

Gata3 Is Required for Reactive Cell Proliferation, Reactive Neurogenesis, and Tissue Distribution of Newborn Neurons(A) Morpholino injection paradigm.(B) Gata3 and BrdU IHC for control morpholino-injected brains.(C) High magnification of the framed region in (B).(D) Gata3 and BrdU IHC for Gata3-antisense morpholino-injected brains.(E) High magnification of the framed region in (D).(F) Quantification graph for BrdU-positive cells in the ventricular region of unlesioned and 3 dpl telencephalic hemispheres.(G) BrdU pulse-chase experiment.(H) BrdU IHC on control morpholino-injected brains.(I) BrdU IHC on Gata3 antisense morpholino-injected brains.(J) Quantification graph. Scale bars, 50 μm. n = 7 fish for (B–F) and 4 for (G–J). Data are represented as mean ± SEM.(K) Morpholino-injection and BrdU pulse-chase schemes for reactive neurogenesis assay. (K1–K3) HuC/D-BrdU double IHC on sham/uninjected, control morpholino-injected, and gata3 morpholino-injected telencephalons.(L) Quantification graph.(M) Morpholino-injection and BrdU pulse-chase schemes for reactive neurogenesis assay for PVA neuronal subtype. (M1–M3) PVA-BrdU double IHC on sham/uninjected, control morpholino-injected, and gata3 morpholino-injected telencephalons.(N) Quantification graph.(O) Morpholino-injection and BrdU pulse-chase schemes to assay for migration of newborn neurons. (O1 and O2) HuC/D-BrdU double IHC on control morpholino-injected and gata3 morpholino-injected telencephalons. (O3 and O4) High-magnification images of BrdU staining in framed regions of (E1 and E2). Dashed line is the ventricular surface.(P) Histogram and bar graph depicting the relative distance distribution of HuC/D-BrdU positive newborn neurons from the ventricle and the average distance migrated by a newborn neuron in control- and antisense morpholino-injected telencephalons. Scale bars: 20 μm (K1–K3, insets of M1–M3, O3, and O4) and 50 μm (M1–M3, O1, and O2). n = 8 fish for (K-L), 6 for (M–N), and 4 for (O–P). Data are represented as mean ± SEM.See also Figures S2 and S3, and Tables S1 and S2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-gata3
Condition:	physical alteration: anatomical structure
Knockdown Reagent:	MO4-gata3
Observed In:	regenerating tissue cell population proliferation regenerating tissue neurogenesis
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 23(6), Kizil, C., Kyritsis, N., Dudczig, S., Kroehne, V., Freudenreich, D., Kaslin, J., and Brand, M., Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3, 1230-1237, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell