FIGURE

Fig. 4

ID

ZDB-FIG-130128-4

Publication

Torregroza et al., 2012 - Regulation of a Vascular Plexus by gata4 Is Mediated in Zebrafish through the Chemokine sdf1a

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Fig. 4

The CHT in gata4 morphants shows aberrant expression of runx1 and osteopontin.

A-D: Shown are representative control (left panels) or gata4 morphant embryos following in situ hybridization to detect expression of runx1 at 48 hpf (A-B) or spp1 at 36 hpf (C-D). A′-D′ are higher magnification views of the tails from panels A-D, respectively. Asterisks in B′ indicate the presence of remaining runx1+ cells associated with the dorsal aorta in morphants. The strong expression in the tails of runx1 and spp1, indicated by the arrows, was reproducibly noted in 60% and 50% of gata4 morphants, respectively (n = 20 each per experiment). Views are lateral, anterior to the left.

Expression Data

Genes:	runx1 spp1
Fish:	WT WT + MO1-gata4
Knockdown Reagent:	MO1-gata4
Anatomical Terms:	dorsal aorta hematopoietic stem cell hematopoietic stem cell intermediate cell mass of mesoderm intermediate cell mass of mesoderm hematopoietic stem cell
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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